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stim1 deltak fragment  (Addgene inc)


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    Structured Review

    Addgene inc stim1 deltak fragment
    Stim1 Deltak Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stim1+yfp/pEX-YFP-STIM1-deltaK+(Plasmid+%2318861)/pm40966085-404-8-11
    Average 92 stars, based on 8 article reviews
    stim1 deltak fragment - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Transfection:

    Article Title: 2,6-Difluorobenzamide derivatives as store-operated calcium channel (SOC) inhibitors.
    Article Snippet: The Ca2+ entry from store-operated Ca2+ channel (SOC) is involved in regulating colorectal cancer progression, such as cell migration.. SOC activation is due to STIM1 translocation and interaction with Orai1 upon Ca2+ depletion in the ER.. Numerous SOC inhibitors, like 2-APB, have been developed and demonstrated their inhibition effects in the preclinical stage.

    Article Title: Aspirin and Sulindac act via different mechanisms to inhibit store-operated calcium channel: Implications for colorectal cancer metastasis.
    Article Snippet: .. STIM1-YFP (Addgene, #19754) and Orai1-mCherry plasmids were transfected into cells using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019). .. Following transfection, the cells were cultured for 24 h on Lab-Tek chambered coverglass (Thermo Fisher Scientific, NUC155383) before NSAID pretreatment.

    Article Title: Trogocytosis of cancer-associated fibroblasts promotes pancreatic cancer growth and immune suppression via phospholipid scramblase anoctamin 6 (ANO6)
    Article Snippet: .. Transfection of GFP-MAPPER (117721, Addgene, Watertown, MA), mCherry-ANO6 (62554, Addgene, Watertown, MA) or STIM1-YFP (19754, Addgene, Watertown, MA) were done by mixing 2.5 μg of plasmid DNA with 7.5 μl of TransIT-X2 reagent (Mirus, Madison, WI). ..

    Generated:

    Article Title: The mammalian trafficking chaperone protein UNC93B1 maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortex.
    Article Snippet: .. The STIM1-TM4-YFP was generated using Gibson Assembly (catalog no.: E2621S; NEB) of fragment 1, made by PCR of STIM1-YFP with the primers 50-TCTGGTCTATCGTTACTCCAAGGAGCAC-30 and 50-CAATGATGGTGTCCTTGAGGTGATTATGG-30, and fragment 2, made by PCR of TLR4-YFP (catalog no.: 13018; Addgene) with primers 50-CCTCAAGGACACCATCATT GGTGTGTC G-30 and 50-TGGAGTAACGATAGACCAG AACTGCTACAAC-3’. ..

    Polymerase Chain Reaction:

    Article Title: The mammalian trafficking chaperone protein UNC93B1 maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortex.
    Article Snippet: .. The STIM1-TM4-YFP was generated using Gibson Assembly (catalog no.: E2621S; NEB) of fragment 1, made by PCR of STIM1-YFP with the primers 50-TCTGGTCTATCGTTACTCCAAGGAGCAC-30 and 50-CAATGATGGTGTCCTTGAGGTGATTATGG-30, and fragment 2, made by PCR of TLR4-YFP (catalog no.: 13018; Addgene) with primers 50-CCTCAAGGACACCATCATT GGTGTGTC G-30 and 50-TGGAGTAACGATAGACCAG AACTGCTACAAC-3’. ..

    Expressing:

    Article Title: Extracellular cysteines C226 and C232 mediate hydrogen sulfide-dependent inhibition of Orai3-mediated store-operated calcium entry.
    Article Snippet: The FRET control construct pECFP18aaEYFP was a gift from Gabriele Kaminski Schierle (Addgene plasmid No. 109330; http://n2t. net/addgene:109330; RRID:Addgene_109330). .. We obtained the expression constructs CFP-Orai3 from James Putney (6); STIM1-YFP from Anjano Rao (addgene plasmid No. 19754) (4); Orai1-GFP and Orai3-GFP from Barbara Niemeyer (28); STIM1mCherry from Richard Lewis (3), Orai3-YFP from Christoph Romanin (29), and mCherry-Orai3 from Trevor Shuttleworth (30). ..

    Construct:

    Article Title: Extracellular cysteines C226 and C232 mediate hydrogen sulfide-dependent inhibition of Orai3-mediated store-operated calcium entry.
    Article Snippet: The FRET control construct pECFP18aaEYFP was a gift from Gabriele Kaminski Schierle (Addgene plasmid No. 109330; http://n2t. net/addgene:109330; RRID:Addgene_109330). .. We obtained the expression constructs CFP-Orai3 from James Putney (6); STIM1-YFP from Anjano Rao (addgene plasmid No. 19754) (4); Orai1-GFP and Orai3-GFP from Barbara Niemeyer (28); STIM1mCherry from Richard Lewis (3), Orai3-YFP from Christoph Romanin (29), and mCherry-Orai3 from Trevor Shuttleworth (30). ..

    Plasmid Preparation:

    Article Title: Extracellular cysteines C226 and C232 mediate hydrogen sulfide-dependent inhibition of Orai3-mediated store-operated calcium entry.
    Article Snippet: The FRET control construct pECFP18aaEYFP was a gift from Gabriele Kaminski Schierle (Addgene plasmid No. 109330; http://n2t. net/addgene:109330; RRID:Addgene_109330). .. We obtained the expression constructs CFP-Orai3 from James Putney (6); STIM1-YFP from Anjano Rao (addgene plasmid No. 19754) (4); Orai1-GFP and Orai3-GFP from Barbara Niemeyer (28); STIM1mCherry from Richard Lewis (3), Orai3-YFP from Christoph Romanin (29), and mCherry-Orai3 from Trevor Shuttleworth (30). ..

    Article Title: Feedback modulation of Orai1α and Orai1β protein content mediated by STIM proteins.
    Article Snippet: .. CMV‐promoter STIM1‐D76A, STIM1‐YFP, STIM2‐YFP, STIM1‐mCherry and STIM2‐mCherry were a gift from Christoph Romanin (Institute of Biophysics, Johannes Kepler University Linz). pEX‐CMV‐SP‐YFP‐STIM2(15‐746) EF hand D80A was a gift from Tobias Meyer (Addgene plasmid # 18864; http://n2t.net/addgene: 18864; RRID:Addgene_18864). ..

    Article Title: Feedback modulation of Orai1α and Orai1β protein content mediated by STIM proteins
    Article Snippet: .. CMV‐promoter STIM1‐D76A, STIM1‐YFP, STIM2‐YFP, STIM1‐mCherry and STIM2‐mCherry were a gift from Christoph Romanin (Institute of Biophysics, Johannes Kepler University Linz). pEX‐CMV‐SP‐YFP‐STIM2(15‐746) EF hand D80A was a gift from Tobias Meyer (Addgene plasmid # 18864; http://n2t.net/addgene:18864 ; RRID:Addgene_18864). ..

    Article Title: Trogocytosis of cancer-associated fibroblasts promotes pancreatic cancer growth and immune suppression via phospholipid scramblase anoctamin 6 (ANO6)
    Article Snippet: .. Transfection of GFP-MAPPER (117721, Addgene, Watertown, MA), mCherry-ANO6 (62554, Addgene, Watertown, MA) or STIM1-YFP (19754, Addgene, Watertown, MA) were done by mixing 2.5 μg of plasmid DNA with 7.5 μl of TransIT-X2 reagent (Mirus, Madison, WI). ..

    other:

    Article Title: STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons
    Article Snippet: STIM1‐YFP , Addgene , 18857.



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